吸光光度法测定铁

吸收曲线的测定

表1 不同波长下测得吸光度
波长/nm	吸光度
450	.190
455	.198
460	.206
465	.215
470	.224
475	.230
480	.234
485	.235
490	.237
495	.240
500	.244
505	.249
510	.252
515	.247
520	.236
525	.213
530	.190
535	.162
540	.128

set terminal svg background "white"
set style line 1 \
    linecolor rgb '#0060ad' \
    linetype 1 linewidth 2 \
    pointtype 7 pointsize 1.5
plot data using 1:2 title 'points' with linespoints linstyle 1

标准曲线的测定

表2 不同浓度下吸光度校正值
浓度 ug/mL	吸光度校正值
0	.000
0.4	.088
0.8	.175
1.2	.250
1.6	.332
2.0	.410

set terminal svg background "white"
f(x)=a*x+b
fit f(x) data u 1:2 via a,b
func=sprintf("y=%.3f*x+%.3f",a,b)
set key top left
plot f(x) title func, data using 1:2 title 'points'